32520170255 AM Q798100 G 3222017 o743 PM Print Calculator Pe

3/25/201702:55 AM Q798/100 G 3/22/2017 o7:43 PM Print Calculator Periodic Table Question 39 of 40 Sapling Learning Mapa\" used to seguendo genomic DNA Amange the seps of whole-genome shotgun sequencing in order from first to last First step Last step isolate genomic DNA Break DNA large fragments that are o base pairs in leng Break DNA into fragments that are Insert DNA into BACs approximately 1000 base pairs in length. Insert DNA into plasmids. Sequence DNA Computationally assemble sequences fom each BAC Computasonally assemble the genome from overlapping BACs. Answer Next Exit PrtScn End

Solution

1.Isolate genomic DNA

2. Break DNA into large fragmnets that are approximately 150,000 bp in length.

3. insert DNA in to BAC.

4. Break DNA into smaller fragmnets that are approximately 1000 bp in length.

5. insert DNA into plasmid.

6. sequence DNA

7. computational assembly of sequence from each BAC

8. Computationally assemble the genome from overlapping BACs