Calculate the concentration of plasmid DNA in gL in your fin

Calculate the concentration of plasmid DNA (in g/L) in your final solution if, hypothetically, you measure A260 0.10 for the sample in the cuvette. Once you measure A260, be ready to calculate the concentration of plasmid DNA in your stock solution

Solution

Answer: 5ug/mL

DNA concentration is estimated by measuring the absorbance at 260nm, multiplying by the dilution factor, and using the relationship that an A260 of 1.0 = 50µg/ml pure dsDNA.

Given: A260 = 0.10

A260 of 1.0 = 50µg/ml pure dsDNA

Therefore 0.1 absorbance corresponds to = 5ug/mL