1What did you learn about the properties of DNA when you hav

1.What did you learn about the properties of DNA when you have isolated DNA from onion. 2.List three important uses of genomic DNA in the lab. 3.You are ask to determine DNA content of a liver tissue Describe how you will proceed in finding amount of DNA in the sample.

Solution

Ques-1: What did you learn about the properties of DNA when you have isolated DNA from onion

Answer: In this laboratory extraction of DNA, I have isolated diced onions into the beaker followed by detergent & salt addition (adequate measurement) to remove cell membrane proteins and lipids. It has observed that deoxyribonucleic acid is insoluble in ice-cold ethanol result in formation of yellow precipitate at the bottom. DNA forms a complex precipitate finally; its double helical structure can be unraveled under the microscope using slide preparation. It has observed that DNA is a polynucleotide polymer

Ques-2: List three important uses of genomic DNA in the lab

Answer: Genomic DNA is useful for whole-genome sequencing of organisms inside the lab

Extracted genomic DNA is useful to compare various genome levels of other divergent species inside the lab to reveal evolutionary aspects

Extracted genomic DNA can be used as reference to construct phylogenetic trees

Genomic DNA is extracted & useful to test pre-natal diagnosis of genetic diseases of developing embryo during genetic screening

Genomic DNA is also useful to study single nucleotide polymorphism in lab & to study forensic genetics