If the DNA sequence of a bacterial genome is 45 x 106 bp in

If the DNA sequence of a bacterial genome is 4.5 x 10^6 bp in size, and the nucleotide sequence is randomly determined, how many times would you expect to find the EcoRI recognition sequence in this genome?

Solution

EcoR1 site is hexamer, GAATTC.

Use the formula: size of DNA * (1/4)^6

=4.5* 10^6 * 1^6) / 4^6

4^6= 4096

= 4500000/4096

= 1098.6 times EcoRI recognition sequence will be present in this genome.