What is the optimal temperature for Taq DNA polymerase A PCR

What is the optimal temperature for Taq DNA polymerase? A PCR primer is 18 bases in length. How many times will it bind to the human genome? (size of human genome is 3.3 times 10^9 bp)

Solution

7. The optimum temprature for activity for Taq polymerase is 75-80 degrees Celcius.This is because Thermus aquaticus is an organism that lives in hot springs and needs a polymerase which can sustain high tempratures.

8. The number of times a primer can bind to the human genome depends on the total number of compatible primer binding sites. Primers are designed based on these sites.