Give a detailed description of FRAPSolutionIt is defined as

Give a detailed description of FRAP.

Solution

It is defined as flouresnce recovery after phto bleaching. It is one of the best methods to see the kinetics of diffusion between tissue and cells.

It is based on the principle that the when the flouresnce of a particular thing is linked or joined by the other cell or tissue it results in the quenching of the flouresnce of that substance. When the non flourscene component is removed again the flouresnce compound regains it flouresnce which could be detected by flouresnce microscopy.

So to determine the contact between two things or to find out the movement of the cell, tissue inside the body thus this method is used..

Eg to determine the movements of phispholipids molecules in the cell membrane