1 You are planning to carry out the Sudan Red test on a set

1. You are planning to carry out the Sudan Red test on a set of 50 samples, but the bottle of Sudan Red dye is several years old. Without wasting a lot of time and effort, how can you be sure that the reagent is still reliable?

2. (A) What is the relationship between albumin and glycine, between starch and glucose, and between triacylglycerols and glycerol? (B) Write a single sentence to express a general rule that explains why the two substance in each pair behave differently in the assays.

Solution

1. Sudan Red dye must be diluted fresh before staining. In case, the dye is old and you need to ascertain whether the dye is reliable, look for the precipitated stain on the wall and neck of the container. In case you find some precipitation, redissolve the precipitated dye in few drops of isopropanol solution.

2.A.

Albumin and glycine: Albumin is a protein and glycine is an amino acid

Starch and glucose: Starch is polysaccharide and glucose is a monosaccharide

Triacylglycerol is an ester obtained when glycerol is bonded with the three fatty acid molecules. Glycerol is a three carbon molecule have 3 hydroxy group attached to it.

B. Biuret test, Iodine test, and Sudan Red test can detect complex organic molecules and not simple organic molecules.